E9379

Stains-All

~95% purity, powder

• Synonyme(s) : 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• Formule empirique (notation de Hill): C₃₀H₂₇BrN₂S₂
• Numéro CAS: 7423-31-6
• Poids moléculaire : 559.58
• Numéro Beilstein : 3865213
• Numéro CE : 231-047-7
• Numéro MDL: MFCD00078788
• Code UNSPSC : 12171500
• ID de substance PubChem : 24894694
• Nomenclature NACRES : NA.47

Propriétés

• product name: Stains-All, ~95%
• Pureté: ~95%
• Niveau de qualité: 200
• Forme: powder
• Solubilité: chloroform: 40 mg (PLUS 4.0 ml)
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-8°C
• Chaîne SMILES: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• Clé InChI: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Description

Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Actions biochimiques/physiologiques

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Attention

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)