T6763
Trypsin Acetylated from bovine pancreas
Type V-S, ≥8,500 BAEE units/mg protein (biuret)
Synonyme(s) :
Acetyltrypsin
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About This Item
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Source biologique
bovine pancreas
Niveau de qualité
Type
Type V-S
Forme
powder
Activité spécifique
≥8,500 BAEE units/mg protein (biuret)
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
cow ... TRYP8(282603)
Catégories apparentées
Description générale
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.
Actions biochimiques/physiologiques
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
Définition de l'unité
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
Notes préparatoires
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
Autres remarques
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
Inhibiteur
Réf. du produit
Description
Tarif
Substrat
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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