The control primers specific for the mouse GAPDH promoter are as follows:
FOR: 5'-CCTCTGCGCCCTTGAGCTAGGA-3’
REV: 5'-CACAAGAAGATGCGGCCGTCTC-3’
RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
Synonyme(s) :
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
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About This Item
Code UNSPSC :
41116158
Nomenclature NACRES :
NA.54
760,00 $
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Description générale
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view application data.
Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Application
Imprint® RNA Immunoprecipitation Kit has been used for:
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques[1]
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Caractéristiques et avantages
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Informations légales
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Réf. du produit
Description
FDS
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderFDS
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderFDS
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionFDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionFDS
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLFDS
En option
Réf. du produit
Description
Tarif
Nécessaire, mais non fourni
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
Code de la classe de stockage
3 - Flammable liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
100.4 °F
Point d'éclair (°C)
38 °C
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Protocoles
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
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Please suggest the appropriate control primer set recommended for use with mouse tissue samples while using EZ Magna Chip A/G kit, 17-10086. It includes control antibodies (anti-RNA pol II) and a control primer set intended for the human GAPDH promoter.
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