If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
M3148
2-mercaptoéthanol
for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Synonyme(s) :
β-mercaptoéthanol, 2-hydroxyéthylmercaptan, BME, Thioéthylèneglycol
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About This Item
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Densité de vapeur
2.69 (vs air)
Pression de vapeur
1 mmHg ( 20 °C)
Gamme de produits
BioReagent
Essai
99% (GC/titration)
Forme
liquid
Limite d'explosivité
18 %
Pertinence de la réaction
reagent type: reductant
Concentration
14.3 M (pure liquid)
Technique(s)
cell culture | mammalian: suitable
electrophoresis: suitable
Indice de réfraction
n20/D 1.500 (lit.)
pH
4.5-6 (20 °C, 500 g/L)
pb
157 °C (lit.)
Solubilité
H2O: soluble 1 mL/mL
Densité
1.114 g/mL at 25 °C (lit.)
Activité étrangère
DNase, RNase, protease, none detected
Température de stockage
2-8°C
Chaîne SMILES
OCCS
InChI
1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
Clé InChI
DGVVWUTYPXICAM-UHFFFAOYSA-N
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Application
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
Organes cibles
Liver,Heart
Code de la classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
154.4 °F - closed cup
Point d'éclair (°C)
68 °C - closed cup
Équipement de protection individuelle
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Certificats d'analyse (COA)
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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
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Les clients ont également consulté
Protocoles
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.
Protocole du test au MTT pour la mesure de la viabilité, de la prolifération et de la toxicité cellulaires. Instructions de préparation du réactif au MTT et exemples d'applications.
Le dosage colorimétrique au service de la quantification non radioactive de la prolifération, de la viabilité et de la toxicité cellulaires sur cellules adhérentes ou en suspension cultivées sur des microplaques de 96 puits.
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How can I determine the shelf life / expiration / retest date of this product?
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Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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I’ve purchased 25mL bottle and want to use this in my cell culture media at concentration of 50mM. What is the concentration in the 25mL bottle and how to make this to 50mM concentration?
1 answer-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
To prepare a 50 mM solution, please mix 0.391 g of M3148 with 100 mL solvent. 0.391 g / 78.13 g/mol / 0.1 L = 0.05 = 50 mM.Helpful?
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Hi, What is the concentration of 2-mercaptoethanol in the 100mL bottle?
1 answer-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
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Hi is this sterile?
1 answer-
This product is not sterile, but can be used in cell culture applications and is DNase, RNase, and protease free.
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