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D9564

Sigma-Aldrich

DAPI, dilactate

≥98% purity (HPLC), powder

Synonyme(s) :

4′,6-Diamidino-2-phenyindole, dilactate

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About This Item

Formule empirique (notation de Hill):
C16H15N5 · 2C3H6O3
Poids moléculaire :
457.48
Code UNSPSC :
12171500
ID de substance PubChem :
329798905
Nomenclature NACRES :
NA.47

product name

DAPI, dilactate, ≥98% (HPLC)

Niveau de qualité

300

Pureté

≥98% (HPLC)

Forme

powder

Couleur

yellow

Solubilité

H2O: 20 mg/mL

ε (coefficient d'extinction)

28.00-32.50 at 345-351 nm in methanol

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Chaîne SMILES 

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

Clé InChI

LCAUPANIXBMNIA-UHFFFAOYSA-N

Catégories apparentées

Description générale

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Actions biochimiques/physiologiques

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000384458
0000384458
0000352352
0000322467
0000300659

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Shahrzad Bahrampour et al.
PLoS biology, 17(2), e3000163-e3000163 (2019-02-27)
During central nervous system (CNS) development, genetic programs establish neural stem cells and drive both stem and daughter cell proliferation. However, the prominent anterior expansion of the CNS implies anterior-posterior (A-P) modulation of these programs. In Drosophila, a set of
Expression and high glucose-mediated regulation of K+ channel interacting protein 3 (KChIP3) and KV4 channels in retinal Muller glial cells.
Chavira-Suarez E, et al.
Biochemical and Biophysical Research Communications, 404, 678-683 (2011)
Vicky Goler-Baron et al.
PloS one, 6(1), e16007-e16007 (2011-02-02)
Multidrug resistance (MDR) is a major impediment to curative cancer chemotherapy. The ATP-Binding Cassette transporters ABCG2, ABCB1 and ABCC2 form a unique defense network against multiple structurally and functionally distinct chemotherapeutics, thereby resulting in MDR. Thus, deciphering novel mechanisms of
Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain.
Vigano F, et al.
Nature Neuroscience, 16, 1370-1372 (2013)
Silvia S Kang et al.
The Journal of experimental medicine, 208(4), 747-759 (2011-04-06)
After virus infection, cytotoxic T lymphocytes (CTLs) divide rapidly to eradicate the pathogen and prevent the establishment of persistence. The magnitude of an antiviral CTL response is thought to be controlled by the initiation of a cell cycle program within
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