10236276001
Roche
4′,6-Diamidino-2-phénylindole dihydrochloride
4′,6-Diamidine-2′-phenylindole dihydrochloride
Synonyme(s) :
2-(4-Amidinophényl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
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About This Item
Formule empirique (notation de Hill):
C16H15N5 · 2HCl
Numéro CAS:
Poids moléculaire :
350.25
Numéro Beilstein :
4894417
Numéro MDL:
Code UNSPSC :
41116100
ID de substance PubChem :
Produits recommandés
Niveau de qualité
Pureté
>90%
Forme
powder
Conditionnement
pkg of 10 mg
Fabricant/nom de marque
Roche
λmax
340 nm in aq. suspension
Fluorescence
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
Température de stockage
room temp
Chaîne SMILES
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
Clé InChI
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Catégories apparentées
Application
Le DAPI est nettement plus sensible que le bromure d′éthidium pour marquer l′ADN dans les gels d′agarose. Il peut être utilisé dans le photofootprinting de l′ADN, pour détecter les sondes hybridées dans les applications sur membranes en permettant la visualisation spécifique des complexes double-brins, et pour évaluer les changements de l′ADN et de son contenu lors de l′apoptose par cytométrie en flux. La coloration par DAPI est également une méthode sensible et spécifique de détection des mycoplasmes.
DAPI is a fluorescent dye that binds selectively to double-stranded DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy.
Actions biochimiques/physiologiques
Sonde fluorescente pour l′ADN qui se lie dans le petit sillon, capable de pénétrer dans les cellules. Se lie au petit sillon d′ADN double brin (préférentiellement à l′ADN riche en AT), formant un complexe stable dont l′intensité de fluorescence est environ 20 fois plus élevée que celle du DAPI non lié.
Qualité
Purity: >90% (from N)
Principe
The fluorescent dye DAPI binds selectively to DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces.
Notes préparatoires
Working solution: Solubility: 25 mg/ml in water
Preparation of stock solution
Dissolve in double-dist. water to a final concentration of 1 to 5 mg/ml.
Note: Do not use any buffers.
Preparation of working solution
Dilute the stock solution with methanol to a final concentration of 1 μg/ml. The working solution is stable at 2 to 8 °C for about 6 months.
Storage conditions (working solution): Stock solution (1 to 5 mg/ml) at -15 to -25 °C for 12 months.
Working solution (1μg/ml) at 2 to 8 °C for about 6 months.
Preparation of stock solution
Dissolve in double-dist. water to a final concentration of 1 to 5 mg/ml.
Note: Do not use any buffers.
Preparation of working solution
Dilute the stock solution with methanol to a final concentration of 1 μg/ml. The working solution is stable at 2 to 8 °C for about 6 months.
Storage conditions (working solution): Stock solution (1 to 5 mg/ml) at -15 to -25 °C for 12 months.
Working solution (1μg/ml) at 2 to 8 °C for about 6 months.
Reconstitution
In 2 to 10 ml double-dist. water; 1 to 5 mg/ml final concentration.
Note: Prepare aliquots and store at -15 to -25 °C.
Note: Prepare aliquots and store at -15 to -25 °C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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