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HPLC Analysis of Tryptic Digest on BIOshell A160 Peptide C18

HPLC Analysis of Tryptic Digest on BIOshell A160 Peptide C18 application for HPLC

Materiais

coluna analítica

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Preços

Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

padrão

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Preços

Casein from bovine milk

suitable for substrate for protein kinase (after dephosphorylation), purified powder

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 0.1% formic acid in water; [B] 25:75, (0.4% formic acid in water):acetonitrile

gradient

0 to 70% B in 40 min.

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+), TOF

injection

2 μL

sample

10 pg/L in 0.1% formic acid

Descrição

Informações legais

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