HPLC Analysis of Substituted Poly Aromatic Hydrocarbons using Ascentis® Express Biphenyl
CONDITIONS
column
Ascentis Express Biphenyl, 10 cm x 2.1 mm, 2.7 μm particles (64065-U)
mobile phase
[A] Water; [B] Methanol; (30:70 A:B)
flow rate
0.5 mL/min
pressure
6200 psi (427 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
1 μL
sample
Uracil (10 μg/mL), Acenaphthenol (250 μg/mL), Acenaphthenone (20 μg/mL),
Acenaphthene (500 μg/mL), Prepared in 70:30 A:B
Descrição
Nota de análise
This application demonstrates the suitability of Ascentis Express Biphenyl for the separation of substituted PAHs. It shows the suitability of this phase for separations of extended aromatics and their oxygenated counter parts. These compounds are potentional metabolites, used as synthetic intermediates, and in the study of dihydrodiol dehydrogenase activity in celluar systems.
Informações legais
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany