HPLC Analysis of Nucleobases using TSKgel^® SuperSW mAb HTP column in HILIC mode

column

TSKgel SuperSW mAb HTP 15 cm x 4.6 mm I.D., 4 μm particles (822855)

column

TSKgel Amide-80 10 cm x 2 mm I.D., 5 μm particles (819695)

mobile phase

HILIC mode: [A]: acetonitrile (HILIC mode); [B]: 15 mmol/L ammonium bicarbonate, pH 7.4 (HILIC mode)

mobile phase

SEC conditions: 100 mmol/L phosphate/100 mmol/L sodium sulfate, pH 6.7 + 0.05% NaN₃ (SEC mode)

gradient

Isocratic

flow rate

0.4 mL/min

column temp.

Ambient

detector

UV, 280 nm

injection

1 μL

sample

uracil (1.5 mg/mL), adenine (1.5 mg/mL), cytosine (1.5 mg/mL), cytidine (1.5 mg/mL)