Pular para o conteúdo
Merck

HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide

HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide application for HPLC

Materiais

Padrão

Nº do produto
Descrição
Preços

S2626

Spermidine

≥99% (GC)

T90344

Tyramine

≥98.0%

55513

Spermine

analytical standard

51799

Putrescine

analytical standard

59964

Histamine

analytical standard

52063

Cadaverine

analytical standard

coluna analítica

Nº do produto
Descrição
Preços

565305-U

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 15 cm × 2.1 mm

CONDITIONS

column

Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)

mobile phase

[A] 0.1% ammonium acetate in water (pH unadjusted); [B] acetonitrile

gradient

25 to 75% B in 10 min; held at 75% B for 5 min

flow rate

0.5 mL/min

column temp.

35 °C

detector

UV, 340 nm

injection

10 μL

Descrição

Nota de análise

Following pre-column derivatization of primary amino groups using o-phthaldialdehyde/thiol reagent, the Ascentis RP-Amide was used for the analysis of biogenic amines commonly found in wines. These include histamine, spermine, spermidine, tyramine, putrescine and cadaverine. Structures along with an optimized chromatogram are presented.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS