HPLC Analysis of Antihypertensive ACE Inhibitors on Discovery^® RP-AmideC16

column

Discovery RP-AmideC16, 15 cm × 4.6 mm I.D., 5 μm particles (505013)

mobile phase

[A] acetonitrile; [B] 25 mM potassium phosphate, pH 2.3 (33:67, A:B)

flow rate

0.6 mL/min

column temp.

35 °C

detector

UV, 214 nm

injection

3 μL