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Merck

Feasibility of a facile butanol bioproduction using planetary mill pretreatment.

Bioresource technology (2015-09-16)
Jeong Heo Kwon, Hyunsoo Kang, Byoung-In Sang, Yunje Kim, Jiho Min, Robert J Mitchell, Jin Hyung Lee
RESUMO

A facile butanol bioproduction process was developed using planetary milling, and Pinus rigida wood waste as a model substrate for fermentable sugars. The use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. Moreover, using this pretreatment process resulted in the production of only 0.072 ± 0.003 g/L soluble phenolic compounds, a concentration that was not inhibitory towards Clostridium beijerinckii NCIMB 8052. As the milling was performed in a compatible buffer (50mM acetate, pH 4.8), the enzymatic hydrolysis step was initiated by simply adding the cellulase cocktail powder directly to pretreated biomass without washing the biomass or exchanging the buffer, resulting in a glucose yield of 31 g/L (84.02%). Fermentation of the hydrolysate samples by C. beijerinckii NCIMB 8052 gave slightly better butanol yields than cultures grown in a typical lab media (P2), with final concentrations of 6.91 and 6.66 g/L, respectively.

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Sigma-Aldrich
Ácido acético, glacial, ACS reagent, ≥99.7%
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Ácido acético, glacial, ReagentPlus®, ≥99%
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Sacarose, for molecular biology, ≥99.5% (GC)
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Acetato de sódio, anhydrous, ReagentPlus®, ≥99.0%
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1-Butanol, 99.9%
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Sacarose, ≥99.5% (GC)
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Acetato de sódio, ACS reagent, ≥99%
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1-Butanol, ACS reagent, ≥99.4%
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Acetato de sódio, ACS reagent, ≥99.0%
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Sacarose, ≥99.5% (GC), BioXtra
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Acetato de sódio, puriss. p.a., ACS reagent, reag. Ph. Eur., anhydrous
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Ácido acético, glacial, ≥99.99% trace metals basis
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Ácido acético, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
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Sacarose, BioUltra, for molecular biology, ≥99.5% (HPLC)
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Ácido acético, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
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Acetic acid solution, suitable for HPLC
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D-(+)-Galactose, ≥99% (HPLC), BioReagent, suitable for cell culture, suitable for insect cell culture
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Acetato de sódio, ReagentPlus®, ≥99.0%
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Butyric acid, ≥99%
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3,5-Dinitrosalicylic acid, used in colorimetric determination of reducing sugars
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Sacarose, ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture
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1-Butanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
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3,5-Dinitrosalicylic acid, 98%
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Sacarose, ≥99.5% (GC)
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Sacarose, ≥99.5% (GC), Grade II, suitable for plant cell culture
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D-(+)-Manose, powder, BioReagent, suitable for cell culture
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Ácido acético, for luminescence, BioUltra, ≥99.5% (GC)
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Acetato de sódio, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E262, 99.0-101.0% (calc. to the dried substance), ≤0.00002% Al
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Acetato de sódio, >99%, FG
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