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822854

TSKgel® SuperSW mAb HR (4 µm) HPLC Column

L x I.D. 30 m × 7.8 mm, diol phase

Sinônimo(s):

TSKgel® Size Exclusion (SW-Type) HPLC Column

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About This Item

Código UNSPSC:
41115711

Nome do produto

Coluna de HPLC de exclusão por tamanho (tipo SW) TSKgel®, phase diol, L × I.D. 30 m × 7.8 mm, 4 μm particle size

Materiais

stainless steel

Agency

suitable for USP L20
suitable for USP L59

linha de produto

TSKgel®

fabricante/nome comercial

Tosoh SuperSW mAb HR, 22854

Parâmetros

≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure

técnica(s)

HPLC: suitable

C × D.I.

30 m × 7.8 mm

Matriz

silica particle platform

Grupo ativo da matriz

diol phase

tamanho de partícula

4 μm

tamanho de poro

250 Å pore size

pH operacional

2.5-7.5

técnica de separação

size exclusion (SEC)

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Descrição geral

The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations.

Aplicação

TSKgel® superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).

forma física

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Informações legais

TSKgel is a registered trademark of Tosoh Corporation

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Certificados de análise (COA)

Lot/Batch Number

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Alexander Rau et al.
mAbs, 13(1), 1902034-1902034 (2021-03-24)
Dual targeting of surface receptors with bispecific antibodies is attracting increasing interest in cancer therapy. Here, we present a novel bivalent and bispecific antagonistic molecule (Dab-Fc) targeting human epidermal growth factors 2 and 3 (HER2 and HER3) derived from the
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Alexander Rau et al.
Molecular cancer therapeutics, 19(7), 1474-1485 (2020-05-21)
The frequent activation of HER3 signaling as a resistance mechanism to EGFR-targeted therapy has motivated the development of combination therapies that block more than one receptor tyrosine kinase. Here, we have developed a novel tetravalent, bispecific single-chain diabody-Fc fusion protein

Artigos

Columns and Analytical Standards for Protein SEC

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

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