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58354

Supelco

SUPELCOSIL LC-8-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
Preço e disponibilidade não estão disponíveis no momento.

Nome do produto

Coluna de HPLC LC-8-DB SUPELCOSIL, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L7

Características

endcapped

fabricante/nome comercial

SUPELCOSIL

Extensão da rotulagem

6.0% carbon loading

Parâmetros

0-70 °C temperature
400 bar pressure (5801 psi)

técnica(s)

HPLC: suitable

C × D.I.

25 cm × 4.6 mm

área da superfície

170 m2/g

cobertura de superfície

surface coverage 3.2 μmol/m2

Matriz

silica gel, spherical particle platform

Grupo ativo da matriz

C8 (octyl) phase

tamanho de partícula

5 μm

tamanho de poro

120 Å

aplicação(ões)

food and beverages

técnica de separação

reversed phase

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Descrição geral

As fases LC-DB SUPELCOSIL são especialmente desativadas para compostos básicos. Essas colunas promovem menos retenção, melhor formato de pico e maior eficiência para bases orgânicas que o que se pode obter com outras colunas de fase reversa convencionais.

Produtos recomendados

Descubra os reagentes LiChropur ideais para análise por HPLC ou LC-MS

Informações legais

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Vesna Cerkvenik-Flajs et al.
Analytica chimica acta, 663(2), 165-171 (2010-03-09)
An analytical method has been developed for the simultaneous determination of the following endectocide drugs in milk: ivermectin, abamectin, doramectin, moxidectin, eprinomectin, emamectin and nemadectin. Samples were extracted with acetonitrile, purified with solid-phase extraction on a reversed phase C(8), derivatised
J H Nichols et al.
Clinical chemistry, 40(7 Pt 1), 1312-1316 (1994-07-01)
This automated assay determines the concentration of the antidepressant fluoxetine (Prozac) and its active metabolite norfluoxetine in serum by reversed-phase HPLC with spectrophotometric detection. Extraction, injection, and quantification are performed by the Gilson Aspec automated sample handler. The patient's specimen
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 750(1), 41-49 (2001-02-24)
A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions

Questions

1–4 of 4 Questions  
  1. Do you have a cleaning procedure for Supelcosil LC-8DB HPLC column, Product 58354?

    1 answer
    1. Yes.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What does DB stand for in Supelcosil LC-8DB HPLC column, Product 58354?

    1 answer
    1. DB stands for "deactivated base", and refers to the base treatment of the packing in this column. Without this treatment, basic compounds will adsorb to active acidic silanol sites.  If starting a new method, Supelco suggests using a new-technology/pure-silica C8, such as the Ascentis C8.

      Helpful?

  4. Can the Supelcosil LC-8DB HPLC column, Product 58354, be used for acidic compounds?

    1 answer
    1. No, it is base treated, therefore acidic compounds will be adsorbed by the base treatment, resulting is peak tailing.

      Helpful?

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