SRP0273
PDE8A1 Active human
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Sinônimo(s):
PDE8, PDE8A, Phosphodiesterase 8A1
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
Produtos recomendados
fonte biológica
human
recombinante
expressed in baculovirus infected insect cells
Ensaio
≥70% (SDS-PAGE)
Formulário
aqueous solution
atividade específica
≥437 pmol/min-μg
peso molecular
119 kDa
embalagem
pkg of 10 μg
concentração
>0.02 mg/mL
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... PDE8A(5151)
Descrição geral
PDE8A (phosphodiesterase 8A) is one of the two subfamilies of PDE8 protein family, the other being PDE8B. PDE8 shows wide range of tissue expression, and is especially abundant in testis. PDE8A contains a Per, ARNT and Sim (PAS) signaling domain in its N-terminal. PDE8s contain receiver (REC) domain, N-glycosylation, N-myristoylation, amidation consensus sites, and potential kinase substrate sites for PKC (protein kinase), PKA and casein kinase.
Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST tag, MW = 119kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST tag, MW = 119kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Aplicação
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Ações bioquímicas/fisiológicas
PDE8 (phosphodiesterase 8) are cAMP-specific enzymes, and they play a role in various processes such as in T-cell activation regulation, chemotaxis of activated lymphocytes, control of testosterone synthesis in Leydig cells, initiation of glucose-induced biphasic insulin response, steroidogenesis, and excitation–contraction coupling. rs2304418 variant in this gene is linked with lower mRNA expression of PDE8A, which in turn is linked with reduced HIV-1 (human immunodeficiency virus) replication. The expression of this protein is significantly high in macrophages, and is controlled by miR-145-5p. PDE8A promotes HIV-1 replication in macrophages and this is controlled at the level of reverse transcription.
Definição da unidade
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C
forma física
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Nota de preparo
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Polymorphism in HIV-1 dependency factor PDE8A affects mRNA level and HIV-1 replication in primary macrophages.
Bol SM et al
Virology, 420(1), 32-42 (2011)
Refolding and kinetic characterization of the phosphodiesterase-8A catalytic domain.
Yan Z et al
Protein Expression and Purification, 64(1), 82-88 (2009)
Cyclic AMP-specific phosphodiesterase, PDE8A1, is activated by protein kinase A-mediated phosphorylation.
Brown KM et al
Febs Letters, 586(11), 1631-1637 (2012)
Thijs Booiman et al.
PloS one, 9(10), e109673-e109673 (2014-10-09)
HIV-1 infected macrophages play a key role in HIV-1 infection. Even during anti-retroviral treatment, macrophages keep producing virus due to suboptimal tissue penetration and reduced efficacy of antiretrovirals. It is therefore of major importance to understand which host factors are
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