SRP0241

EGFR (T790M) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Sinônimo(s): ERBB1, Epidermal growth factor receptor, HER1, PIG61, Proto-oncogene c-ErbB-1
• Código UNSPSC: 12352200

Propriedades

• fonte biológica: human
• recombinante: expressed in baculovirus infected insect cells
• Ensaio: ≥70% (SDS-PAGE)
• Formulário: aqueous solution
• peso molecular: 88 kDa
• embalagem: pkg of 10 μg
• concentração: >0.02 mg/mL
• nº de adesão NCBI: NM_005228
• nº de adesão UniProt: P00533
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... EGFR(1956)

Descrição

Descrição geral

Human EGFR T790M (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88 kDa, expressed in baculovirus expression system.

Aplicação

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definição da unidade

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.