SAB4200071
ANTI-FLAG® antibody, Rat monoclonal
clone 6F7, purified from hybridoma cell culture
Sinônimo(s):
Anti-ddddk, Anti-dykddddk
Faça loginpara ver os preços organizacionais e de contrato
Selecione um tamanho
200 μL
R$ 2.842,00
R$ 2.842,00
Check Cart for Availability
Selecione um tamanho
Alterar visualização
200 μL
R$ 2.842,00
About This Item
R$ 2.842,00
Check Cart for Availability
Produtos recomendados
fonte biológica
rat
conjugado
unconjugated
forma do anticorpo
purified from hybridoma cell culture
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
6F7, monoclonal
Formulário
buffered aqueous solution
reatividade de espécies
all
técnica(s)
immunoprecipitation (IP): 2.5-5.0 μg using lysates of transiently transfected cells expressing C-terminal-FLAG-tagged protein
western blot: 0.5-1.0 μg/mL using extracts of transiently transfected cells expressing C-terminal-FLAG-tagged protein
Isotipo
IgG1
sequência de imunogênio
(DYKDDDDK)
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Descrição geral
Monoclonal Anti-FLAG® (rat IgG1 isotype) is derived from the hybridoma 6F7 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG® peptide. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Monoclonal Anti-FLAG® recognizes N-terminal,
C-terminal and internal Flag-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG®-tagged fusion proteins.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in associationith other tags.[1] The small size of the FLAG® tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function.
The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage of the C-terminal FLAG® peptide from a fusion protein catalyzed by Cu2+ ions has been reported.[2] A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+dependent protein b-phosphatase[3], as well as in the human and bovine enzyme.
Monoclonal Anti-FLAG® recognizes N-terminal,
C-terminal and internal Flag-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG®-tagged fusion proteins.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in associationith other tags.[1] The small size of the FLAG® tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function.
The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage of the C-terminal FLAG® peptide from a fusion protein catalyzed by Cu2+ ions has been reported.[2] A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+dependent protein b-phosphatase[3], as well as in the human and bovine enzyme.
Imunogênio
FLAG peptide
DYKDDDDK
DYKDDDDK
Aplicação
ANTI-FLAG® antibody, Rat monoclonal has been used in:
- chromatin immunoprecipitation (ChIP)[4]
- western blotting[5]
- coimmunoprecipitation[6][7]
- flow cytometric analysis[7]
Browse additional application references in our FLAG® Literature portal.
Learn more product details in our FLAG® application portal.
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Informações legais
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
10 - Combustible liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
Não está vendo a versão correta?
Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis
Dai L, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 99(6), 736-736 (2019)
Rahul Bhowmick et al.
The Journal of biological chemistry, 287(42), 35004-35020 (2012-08-14)
Viruses have evolved to encode multifunctional proteins to control the intricate cellular signaling pathways by using very few viral proteins. Rotavirus is known to express six nonstructural and six structural proteins. Among them, NSP4 is the enterotoxin, known to disrupt
Xiaolei Li et al.
eLife, 6 (2017-08-19)
Acquired therapeutic resistance by tumors is a substantial impediment to reducing the morbidity and mortality that are attributable to human malignancies. The mechanisms responsible for the dramatic shift between chemosensitivity and chemoresistance in colorectal carcinoma have not been defined. Here
PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wntbeta-catenin signaling pathway
Li Q, et al
Journal of Experimental & Clinical Cancer Research, 36(1), 85-85 (2017)
Rotaviral enterotoxin nonstructural protein 4 targets mitochondria for activation of apoptosis during infection
Bhowmick R, et al.
The Journal of Biological Chemistry, 287(42), 35004-35020 (2012)
Active Filters
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
