R7757
Red Blood Cell Lysing Buffer Hybri-Max™
Liquid, sterile-filtered, suitable for hybridoma
Sinônimo(s):
RBC Lysing Buffer
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
Produtos recomendados
product name
Red Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma
esterilidade
sterile-filtered
forma
liquid
técnica(s)
cell culture | hybridoma: suitable
Impurezas
endotoxin, tested
Aplicação
Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.
Ações bioquímicas/fisiológicas
Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.
Componentes
Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer.
Outras notas
Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher.
Informações legais
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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