R3155

Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal

clone RabT-50, purified from hybridoma cell culture

• Sinônimo(s): Mouse Anti-Rabbit IgG
• Código UNSPSC: 12352203
• NACRES: NA.46

Propriedades

• fonte biológica: mouse
• conjugado: peroxidase conjugate
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: secondary antibodies
• clone: RabT-50, monoclonal
• Formulário: buffered aqueous solution
• reatividade de espécies: rabbit
• não deve reagir com: human, horse, bovine, cat, rat, chicken, pig, goat, monkey
• concentração: 2.5-5.0 mg/mL (antibody)
• técnica(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody; western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.
• Isotipo: IgG1
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).

Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.

Especificidade

Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.

Imunogênio

Enriched fraction of rabbit IgG light chain.

Aplicação

Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• western blotting

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable