PURE1A
Protein A Antibody Purification Kit
sufficient for 10 purifications
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.32
Produtos recomendados
uso
sufficient for 10 purifications
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Descrição geral
The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.
Especificidade
Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Aplicação
PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.
Características e benefícios
- High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
- Specific - will only bind IgG
- Easy to use - antibody is eluted and desalted in a single step, ready to use
- Gentle - avoids prolonged exposure of the antibody to low pH
Princípio
Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.
Exoneração de responsabilidade
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Somente componentes do kit
Nº do produto
Descrição
- Binding Buffer 225 mL
- Desalting Cartridge 1 ea
- Elution Buffer 75 mL
- HEPES Buffer 225 mL
- 5 ml Syringe 1
- 10 ml Syringe 1
- Protein A Cartridge 1 mL
- Regeneration Buffer 75 mL
Ver tudo (8)
produto relacionado
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 4 Oral - STOT SE 1
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Protocolos
Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.
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