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PEROX1

Sigma-Aldrich

Peroxisome Isolation Kit

isolate peroxisomes from tissues and cells

Sinônimo(s):

Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit

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1 KIT
R$ 3.388,00

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1 KIT
R$ 3.388,00

About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

R$ 3.388,00


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Nível de qualidade

técnica(s)

centrifugation: suitable
fractionation: suitable

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

Isolated peroxisomes are used for studying lipid β-oxidation,[1] amino acid metabolism[2] and biosynthesis of ether-linked glycerolipids[3] and bile acids.[4][5]

Aplicação

The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.

Características e benefícios

  • Specially formulated extraction reagents for research scale applications - save time and minimize waste
  • Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
  • Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)

Outras notas

Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.

Informações legais

OptiPrep is a trademark of Serumwerk Bernburg AG

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Ponto de fulgor (°F)

188.6 °F - closed cup

Ponto de fulgor (°C)

87 °C - closed cup


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C M Rodrigues et al.
Journal of lipid research, 37(3), 540-550 (1996-03-01)
We recently demonstrated that the formation of delta 22-bile acids is a quantitatively major pathway for normal bile acid synthesis in the adult male Sprague-Dawley rat. This pathway is specific for 7 beta-hydroxy bile acids and, when ursodeoxycholic acid is
Shurong Hou et al.
SLAS discovery : advancing life sciences R & D, 22(7), 887-896 (2017-03-28)
Primary hyperoxaluria is the underlying cause of oxalosis and is a life-threatening autosomal recessive disease, for which treatment may require dialysis or dual liver-kidney transplantation. The most common primary hyperoxaluria type 1 (PH1) is caused by genetic mutations of a
M Y Kang et al.
Cell death and differentiation, 20(1), 117-129 (2012-08-25)
The tumor suppressor p53 is an important regulator of intracellular reactive oxygen species (ROS) levels, although downstream mediators of p53 remain to be elucidated. Here, we show that p53 and its downstream targets, p53-inducible ribonucleotide reductase (p53R2) and p53-inducible gene
M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates

Artigos

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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