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MITOISO2

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Sinônimo(s):

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

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1 KIT
R$ 2.714,00

R$ 2.714,00

Previsão de entrega em04 de abril de 2025

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1 KIT
R$ 2.714,00

About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

R$ 2.714,00

Previsão de entrega em04 de abril de 2025

Solicite uma grande encomenda

Nível de qualidade

200

uso

sufficient for 50 applications (2-5 x 107 cells)

técnica(s)

ELISA: suitable
western blot: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells.[1] The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Aplicação

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
  • Ewing′s Sarcoma Family of Tumor (ESFT) cells[2]
  • blood samples of tuberculosis patients[3]
  • human neuroblastoma cells and glioma cells[4]
  • myotubes[5]
  • HepG2 cells[6]

Características e benefícios

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

Outras notas

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mLSDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mLSDS
  • C0356Protein Extraction Reagent Type 4 1 bottleSDS

Palavra indicadora

Warning

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

188.6 °F - closed cup

Ponto de fulgor (°C)

87 °C - closed cup

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000354288
0000302541
0000354288
0000232608
0000241135

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Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

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Youguang Gao et al.
Journal of translational medicine, 18(1), 114-114 (2020-03-07)
We have reported that polydatin (PD) alleviates mitochondrial dysfunction in rat models of sepsis-induced acute kidney injury (SI-AKI), but the mechanism is not well understood. Here, we investigated the role of Parkin-mediated mitophagy in the protective effects of PD in
Theodora Panagaki et al.
Biomolecules, 10(4) (2020-04-29)
Down syndrome (trisomy of human chromosome 21) is a common genetic disorder. Overproduction of the gaseous mediator hydrogen sulfide (H2S) has been implicated in the pathogenesis of neurological and metabolic deficits associated with Down syndrome. Several lines of data indicate
Mitochondria
Cooper G M
Journal of biological and chemical chronicles (2000)
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Neoplasia (New York, N.Y.), 11(9), 901-909 (2009-09-03)
At present, there is no efficient curative therapy for cancer patients with advanced metastatic disease. Targeting of antiapoptotic molecules acting on the mitochondrial apoptosis pathway could potentially augment antimetastatic effect of cytotoxic drugs. Similarly to Bcl-2 family members, beta-galactoside-binding lectin
Effects of Moringa oleifera Leaves Extract on High Glucose-Induced Metabolic Changes in HepG2 Cells
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