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P8124

Sigma-Aldrich

Pyoverdines

from Pseudomonas fluorescens, >90% (HPLC)

Sinônimo(s):

Pyoverdine Detection, Pyoverdine Protein, Siderophore Protein

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

fonte biológica

Pseudomonas fluorescens

Nível de qualidade

Ensaio

>90% (HPLC)

forma

powder

solubilidade

H2O: ~10 mg/mL

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

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Aplicação

Pyoverdines from Pseudomonas fluorescens has been used :
  • in tryptophan fluorescence quenching to determine its binding to neutrophil-gelatinase-associated lipocalin (NGAL)
  • to detect pyoverdine diffusion surrounding siderophore-conjugated monobactam (MB-1)-resistant colonies in chelexed, dialyzed Mueller-Hinton broth (CDMHB)
  • for synchronous fluorescence spectra of purified pyoverdine

Ações bioquímicas/fisiológicas

Pyoverdines help in promoting plant growth by chelating iron and rendering it unavailable for plant pathogens. The ferri-pyoverdine complex acts as a signaling molecule inducing the production of secreted virulence factors in Pseudomonas aeruginosa.
Pyoverdines, also called pseuobactins and pyoverdins, are fluorescent siderophores that have high affinity for iron (1032 M-1), and are synthesized by fluorescent pseudomonads under iron-deficient growth conditions. Pyoverdines were shown to prevent iron toxicity produced by iron overload in hepatocyte cultures and effectively scavenges the hydroxyl and peroxyl radicals. Pyoverdines are effective in acquiring iron from transferrin and lactoferrin. Pyoverdines are also involved in the suppression of pythium-induced damping-off of tomato and promotion of growth in some higher plants.

forma física

Supplied as a mixture containing mainly the succinic acid (MW=1161), 2-hydroxy glutaramide (MW=1190), and succinamide (MW=1160) forms of pyoverdines.

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Nº do produto
Descrição
Preços

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Visite a Biblioteca de Documentos

Adaptation-based resistance to siderophore-conjugated antibacterial agents by Pseudomonas aeruginosa.
Tomaras AP
Antimicrobial Agents and Chemotherapy, 57(9), 4197-4207 (2013)
Pyoverdine, the Major Siderophore in Pseudomonas aeruginosa, Evades NGAL Recognition.
Peek ME
Interdisciplinary perspectives on infectious diseases, 843509-843509 (2012)
Correction: Multicolor Whole-Cell Bacterial Sensing Using a Synchronous Fluorescence Spectroscopy-Based Approach.
PLoS ONE (2015)
Biological activity of secondary metabolites produced by a strain of Pseudomonas fluorescens.
Boruah HP and Kumar BS
Folia Microbiologica, 47(4), 359-363 (2002)
Purification of Pyoverdines of Pseudomonas fluorescens 2-79 by Copper-Chelate Chromatography.
Xiao R and Kisaalita WS
Applied and Environmental Microbiology, 61(11), 3769-3774 (1995)

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