P6516

Poli-L-lisina

suitable for cell culture, Mol wt ≤15,000 by MALLS

• Sinônimo(s): L-Lysine homopolymer hydrobromide
• Número CAS: 25988-63-0
• Número MDL: MFCD00237230
• Código UNSPSC: 12352209
• ID de substância PubChem: 24898773
• NACRES: NA.26

Propriedades

• product name: Poli-L-lisina, mol wt 4,000-15,000 by viscosity
• forma: solid
• Nível de qualidade: 200
• peso molecular: ≤15,000 by MALLS; 4,000-15,000 by viscosity
• pontuação do produto alternativo mais ecológico: old score: 51; new score: 43; Find out more about DOZN™ Scoring
• características do produto alternativo mais ecológico: Designing Safer Chemicals; Safer Solvents and Auxiliaries; Reduce Derivatives; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnica(s): cell culture | mammalian: suitable
• cor: white to off-white
• aplicação(ões): cell analysis
• categoria alternativa mais ecológica: , Re-engineered
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• chave InChI: WBSCNDJQPKSPII-KKUMJFAQSA-N

Descrição

Descrição geral

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Designing Safer Chemicals”, "Safer Solvents and Auxiliaries" and “Reduce Derivatives”. Click here to view its DOZN scorecard.

Aplicação

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule. Poly-L-lysine hydrobromide has been used:

• as a component of polyplexes and in DNA condensation experiments
• for coating circular mica disks for atomic force microscopy studies and SuperFrost glass slides for immunofluorescence studies of in HL-60 cells
• as a reference standard for generating calibration curve for the quantification of ε- Poly-L-lysine

Ações bioquímicas/fisiológicas

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Componentes

A poli-L-lisina é um polímero de aminoácido com carga positiva, com cerca de um HBr por resíduo de lisina. O hidrobrometo possibilita que a poli-L-lisina esteja em uma forma cristalina solúvel em água. Uma pequena quantidade do produto pode ser encontrada na estrutura beta, porque o HBr interfere na ligação de hidrogênio entre o grupo amina e o grupo carboxila ou as porções que contêm N ou O.

Atenção

As soluções estéreis são estáveis por até 2 anos se armazenadas a 2 ºC a 8 °C. Deve ser armazenado dessecado a -20 °C.

Nota de preparo

This product has a molecular weight of 4,000-15,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)