P3326

Platelet-Derived Growth Factor-AB human

PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinônimo(s): PDGF-AB
• Número MDL: MFCD00213024
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in E. coli
• Ensaio: ≥97% (SDS-PAGE and N-terminal analysis)
• forma: lyophilized powder
• potência: 10-100 ng/mL ED₅₀/EC₅₀
• qualidade: endotoxin tested
• peso molecular: 27-31 kDa
• embalagem: pkg of 10 μg; pkg of 50 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: ≤1 EU/μg
• nº de adesão UniProt: P04085, P01127
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... PDGFA(5154) , PDGFB(5155)

Descrição

Descrição geral

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.

Aplicação

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

Ações bioquímicas/fisiológicas

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.

Outras notas

Heterodimer of the A and B chains of PDGF disulfide linked.

forma física

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

Nota de análise

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)