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Key Documents

P1034

Sigma-Aldrich

Phenyl isothiocyanate

Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid

Sinônimo(s):

PITC

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About This Item

Fórmula linear:
C6H5NCS
Número CAS:
Peso molecular:
135.19
Beilstein:
471392
Número CE:
Número MDL:
Código UNSPSC:
12352118
ID de substância PubChem:
NACRES:
NA.31

grau

Sigma Grade

Nível de qualidade

Ensaio

≥99% (GC)

forma

liquid

concentração

8.36 M

índice de refração

n20/D 1.6515 (lit.)

pb

218 °C (lit.)

pf

−21 °C (lit.)

densidade

1.132 g/mL at 20 °C (lit.)

adequação

suitable for solid phase protein sequencing analysis

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

S=C=Nc1ccccc1

InChI

1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H

chave InChI

QKFJKGMPGYROCL-UHFFFAOYSA-N

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Descrição geral

Phenyl isothiocyanate belongs to the class of aromatic compounds with isothiocyanate groups. It is typically used as a precolumn derivatization agent for the formation of phenylthiocarbamyl amino acids for their subsequent determination using chromatography techniques.

Aplicação

Phenyl isothiocyanate may be used in the polypeptide and protein sequencing at the subpicomole and subnanomole levels, respectively, by applying automated Edman chemistry followed by the analysis using high-performance liquid chromatography (HPLC) technique.
Derivatizing reagent for primary and secondary amines. Used in sequencing peptides by Edman degradation and in amino acid analyses by HPLC.

Embalagem

One mL size packaged in flame sealed amber ampules under argon.
Ten mL size packaged in screw cap amber vials under argon.

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

190.4 °F - closed cup

Ponto de fulgor (°C)

88 °C - closed cup

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis
Tempst P and Riviere L
Analytical Biochemistry, 183(2), 290-300 (1989)
R L Heinrikson et al.
Analytical biochemistry, 136(1), 65-74 (1984-01-01)
Methods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at
The Protein Protocols Handbook, 235(1), 48-62 (1996)
Additives for High Performance Applications: Chemistry and Applications
Additives for High Performance Applications: Chemistry and Applications, 235(1), 48-62 (2016)
H S Lu et al.
Archives of biochemistry and biophysics, 235(1), 48-62 (1984-11-15)
Automated solid-phase sequencing using 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate (DABITC) double coupling or regular phenylisothiocyanate (PITC) degradation procedures have been investigated. Employing sensitive high-performance liquid chromatography for the identification of amino acid thiohydantoin derivatives (PTH and DABTH), both methods were capable of sequencing

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