NA0300S
GenElute™ HP Plasmid Maxiprep Kit
sufficient for 4 purifications
Sinônimo(s):
GenElute™ HP Plasmid Maxiprep Kit
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About This Item
Código UNSPSC:
41105501
NACRES:
NA.52
Produtos recomendados
uso
sufficient for 4 purifications
características do produto alternativo mais ecológico
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
técnica(s)
DNA purification: suitable
categoria alternativa mais ecológica
temperatura de armazenamento
15-25°C
Descrição geral
The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Aplicação
GenElute™ HP Plasmid Maxiprep Kit has been used to isolate plasmid DNA from Escherichia coli.
Características e benefícios
- From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
- Up to 1.2 mg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- Contains fewer plastic components than other high speed kits, reducing the amount of waste
- No phenol/chloroform extraction or alcohol precipitation required
Outras notas
For additional information, please see www.sigma-aldrich.com/genelutehp.
Informações legais
GenElute is a trademark of Sigma-Aldrich Co. LLC
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Órgãos-alvo
Central nervous system
Código de classe de armazenamento
3 - Flammable liquids
Ponto de fulgor (°F)
77.0 °F - closed cup
Ponto de fulgor (°C)
25 °C - closed cup
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Protocolos
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
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