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NA0160

Sigma-Aldrich

GenElute™ HP Plasmid Miniprep Kit

greener alternative

sufficient for 350 preparations

Sinônimo(s):

GenElute™ HP Plasmid Kit, HP miniprep kit, fast miniprep kit, fast plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation kit, Gen Elute

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.52

uso

sufficient for 350 preparations

características do produto alternativo mais ecológico

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnica(s)

DNA extraction: suitable

categoria alternativa mais ecológica

Aligned,

temperatura de armazenamento

15-25°C

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.

The GenElute™ HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Aplicação

The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transcription, and sequencing.

Características e benefícios

  • Purified plasmid DNA in less than 30 minutes
  • Up to 25 μg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • No phenol/chloroform extraction or alcohol precipitation required

Informações legais

GenElute is a trademark of Sigma-Aldrich Co. LLC

Palavra indicadora

Danger

Frases de perigo

H290,H302,H314,H334,H336

Classificações de perigo

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

Órgãos-alvo

Central nervous system

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Dayanidhi Raman
Methods in Enzymology (2009)
Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype'Calcutta-4'Musa acuminata ssp. burmannicoides
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