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Documentos Principais

N7271

Sigma-Aldrich

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

Sinônimo(s):

Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

Número CAS:
Número da licença da enzima:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

Streptococcus pneumoniae

Nível de qualidade

Formulário

buffered aqueous solution

concentração

≥5 units/mL

atividade externa

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Informações sobre genes

Streptococcus pneumoniae R6 ... nanB(933504)

Ações bioquímicas/fisiológicas

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Embalagem

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Definição da unidade

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

forma física

Solution in 50 mM sodium phosphate, pH 7.5

Nota de preparo

Expressed in glycosidase-free hosts.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Patricia J Campbell et al.
Journal of virology, 88(7), 3802-3814 (2014-01-17)
The 2009 H1N1 lineage represented the first detection of a novel, highly transmissible influenza A virus genotype: six gene segments originated from the North American triple-reassortant swine lineage, and two segments, NA and M, derived from the Eurasian avian-like swine
Alana L Woodward et al.
Veterinary microbiology, 169(3-4), 113-127 (2014-02-01)
Equine influenza viruses are a major cause of respiratory disease in horses worldwide and undergo antigenic drift. Several outbreaks of equine influenza occurred worldwide during 2010-2012, including in vaccinated animals, highlighting the importance of surveillance and virus characterisation. Virus isolates
Tadatsugu Imamura et al.
Journal of virology, 88(5), 2374-2384 (2013-12-29)
Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying
Chinh Tran-To Su et al.
BMC bioinformatics, 14 Suppl 16, S7-S7 (2014-02-26)
Since late March 2013, there has been another global health concern with a sudden wave of flu infections by a novel strain of avian influenza A (H7N9) virus in China. To-date, there have been more than 100 infections with 23
Daniela A Bermejo et al.
Nature immunology, 14(5), 514-522 (2013-04-09)
Here we identified B cells as a major source of rapid, innate-like production of interleukin 17 (IL-17) in vivo in response to infection with Trypanosoma cruzi. IL-17(+) B cells had a plasmablast phenotype, outnumbered cells of the TH17 subset of

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Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

Questions

1–5 of 5 Questions  
  1. What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.

      Helpful?

  2. What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?

    1 answer
    1. The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.

      Helpful?

  5. What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.

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