A6805

β-N-Acetylglucosaminidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• Sinônimo(s): β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase
• Número CAS: 9012-33-3
• Número da licença da enzima: 3.2.1.52 (BRENDA, IUBMB)
• Número CE: 232-730-2
• Número MDL: MFCD00081402
• Código UNSPSC: 12352204
• NACRES: NA.32

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 100
• Formulário: buffered aqueous solution
• atividade específica: ≥80 units/mg protein
• embalagem: vial of ≥1.0 unit
• atividade externa: β-galactosidase, α-mannosidase, α-fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.)
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: Streptococcus pneumoniae R6 ... lytB(934406)

Descrição

Aplicação

β-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I.[1] It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.[2]

Ações bioquímicas/fisiológicas

β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins.[3] It belongs to the glycoside hydrolase family 85 (GH85).[4] Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides[5] and acts on fucosylated N-glycan core.[4] Its deglycosylation functionality is exploited in studying functions of glycoproteins.[5]

Specific for GlcNAc; not significantly active with GalNAc

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

Definição da unidade

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.

forma física

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable