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N3013

Sigma-Aldrich

Nile Red

Powder

Sinônimo(s):

Nile Blue A Oxazone

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100 MG
R$ 1.169,00
1 G
R$ 5.814,00

R$ 1.169,00

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100 MG
R$ 1.169,00
1 G
R$ 5.814,00

About This Item

Fórmula empírica (Notação de Hill):
C20H18N2O2
Número CAS:
7385-67-3
Peso molecular:
318.37
Beilstein:
279110
Número CE:
230-966-0
Número MDL:
MFCD00011639
Código UNSPSC:
12171500
ID de substância PubChem:
24897612
NACRES:
NA.47

R$ 1.169,00

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Nome do produto

Nile Red, Technical grade

grau

technical grade

Formulário

powder

composição

Carbon: 70.0-77.3%

Nitrogen: 7.5-8.8%

técnica(s)

titration: suitable

cor

faint green to brown

pf

203-205 °C (lit.)

solubilidade

methanol: 1 mg/mL

λmax

553 nm

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

chave InChI

VOFUROIFQGPCGE-UHFFFAOYSA-N

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Descrição geral

Nile Red is a lipophilic fluorescent dye that is commonly used in various scientific applications, particularly in lipid research. It has the ability to stain and visualize lipid droplets in cells and tissues.

Aplicação

Nile red is widely employed in studies related to lipid metabolism, lipid droplet dynamics, lipid accumulation in cells or tissues, and lipid-based drug delivery systems.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
SLBP9326V
122K3481
116H3402
089H3626
073H3702

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Zebrafish obesogenic test: a tool for screening molecules that target adiposity.
Tingaud-Sequeira A
Journal of Lipid Research, 52, 1765-1765 (2011)
Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium.
Kosta A
The Journal of Biological Chemistry, 279, 48404-48404 (2004)
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Analytical biochemistry, 167(2), 228-234 (1987-12-01)
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for
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Questions

  1. How should n3013 be dissolved and diluted for lipid staining?

    1 answer

    1. There has not been any conduct suitability testing for Nile Red. Please see the following for an article that may offer information; J. Histochem. Cytochem. 35:619-621 reference.
      To perform the staining, a stock solution of Nile red was prepared by dissolving 10 mg of Nile red in 10 ml of acetone, which was then stored in the refrigerator protected from light. The sections were fixed in 1% glutaraldehyde in PBS for 5 minutes and washed in PBS three times for 2 minutes each. The staining solution was then prepared by diluting 100 ul of the stock solution in 1 ml of PBS. The sections were covered with this solution for 20 minutes at room temperature, then briefly washed in PBS and mounted in 75% glycerol in PBS. Other sections were incubated with solutions containing 50, 10, and 1 ug and 100 ng of Nile red per ml. The sections were examined with a filter set for fluorescein fluorescence (450-500 nm band pass excitation filter, a 510 nm center wavelength chromatic beam splitter, and a 528 nm long-pass barrier filter) and were photographed.

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