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SCT144

Sigma-Aldrich

BioTracker 488 Green Lipid Droplet Dye

Live cell imaging dye that rapidly stains lipid droplets with no wash steps and minimal background staining of other cellular membranes or organelles.

Sinônimo(s):

Live cell imaging probe, Live cell lipid imaging probe

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.47
Preço e disponibilidade não estão disponíveis no momento.

técnica(s)

cell based assay: suitable

método de detecção

fluorometric

Condições de expedição

ambient

Descrição geral

Lipids are fundamental building blocks of cells and play important cellular roles. Lipids are key components of the plasma membrane and other cellular compartments, including the nuclear membrane, endoplasmic reticulum, Golgi apparatus, and trafficking vesicles such as endosomes and lysosomes. Intracellular lipid droplets are cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters. Lipids have been implicated in various diseases including heart, vascular and neurological diseases.

BioTracker Lipid dyes rapidly stain lipid droplets in live or fixed cells with no wash step and minimal background staining of cellular membranes or other organelles. Cells also can be fixed and permeabilized after staining.

The BioTracker 488 Green Lipid Dye has an excitation around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence using the FITC detection channel.

Spectral Properties
Absorbance: 427nm
Emission: 585nm

Aplicação

Live cell fluorescent imaging
Live cell imaging dye that rapidly stains lipid droplets with no wash steps and minimal background staining of other cellular membranes or organelles.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Qualidade

Spectral Properties
Absorbance: 427nm
Emission: 585nm

forma física

Liquid, supplied as a 1000X solution in DMSO

Armazenamento e estabilidade

Store BioTracker 488 Green Lipid Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

188.6 °F - (refers to pure substance)

Ponto de fulgor (°C)

87 °C - (refers to pure substance)

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Questions

1–2 of 2 Questions  

  1. Are there any solutions for the high background issue experienced when using the product with heart tissue cells?

    1 answer

    1. The high background issue could be due to the use of antifade, which is not recommended. Instead, it's suggested to use PBS as the mounting medium and nail polish as the coverslip sealant. Additionally, using other mounting media such as EverBrite™ or FluoroShield™ may impact staining patterns, so it's important to consider the recommended mounting methods to avoid issues with signal intensity and staining patterns.

      Helpful?

  2. Is BioTracker 488 Green Lipid Dye (SCT144) toxic for the cells? Can they be monitored for extra time after staining? How long does the stain last in this case?

    1 answer

    1. No cytotoxicity of this dye has been observed with incubation periods of up to 24 hours. Cytotoxicity beyond the 24-hour incubation period has not been studied. Suitability for extended incubation times would have to be determined by the end user.

      Helpful?

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