M9532
Mannitol Dehydrogenase from Leuconostoc mesenteroides
lyophilized powder, ≥60 units/mg protein
Sinônimo(s):
polyol dehydrogenases, Mannitol:NAD oxidoreductase
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
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Formulário
lyophilized powder
Nível de qualidade
atividade específica
≥60 units/mg protein
peso molecular
136 kDa
temperatura de armazenamento
−20°C
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Descrição geral
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
The molecular weight of mannitol dehydrogenase is 136 kDa.
Aplicação
Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
This preparation is useful in the determination of mannitol in urine.
Ações bioquímicas/fisiológicas
Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.
Definição da unidade
One unit will reduce 1.0 μmole of D-fructose per min in the presence of NADH at pH 5.3 at 30°C. One unit of enzyme, as defined above, is equivalent to approximately 1.8 units of enzyme, as described by Lunn et al., one unit will oxidize 1.0 μmole of D-mannitol per min in the presence of NAD at pH 8.6 at 40°C.
forma física
Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol
Nota de preparo
Partially purified
Nota de análise
Assayed according to the method of Yamanaka, Meth. Enzymol., 41(B), 138 (1975).
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves
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Polyol metabolism in homopterans at high temperatures: accumulation of mannitol in aphids (Aphididae: Homoptera) and sorbitol in whiteflies (Aleyrodidae: Homoptera)
Hendrix D.L. , Salvucci, M.E.
Comparative Biochemistry and Physiology, 120, 487-494 (1998)
Enzymatic production of D-mannitol with the Leuconostoc pseudomesenteroides mannitol dehydrogenase coupled to a coenzyme regeneration system
Parmentier S
Biocatalysis and Biotransformation, 23, 1-7 (2005)
J Aarnikunnas et al.
Applied microbiology and biotechnology, 59(6), 665-671 (2002-09-13)
The N-terminal amino acid sequences of the intact protein and three tryptic peptides from a 41 kDa protein purified from a commercial mannitol dehydrogenase (MDH) enzyme preparation of Leuconostoc mesenteroides ATCC-9135 were determined. Oligonucleotides deduced from these peptide sequences were
Purification and characterization of mannitol dehydrogenase from Aspergillus parasiticus.
Niehaus WG and Dilts RP
Journal of Bacteriology, 151(1), 243-250 (1982)
Purification and characterization of a novel mannitol dehydrogenase from Lactobacillus intermedius
Saha, B.
Biotechnology Progress, 20, 537-542 (2012)
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