product M1255, Calcein Blue to is used to stain cells, but it will stain living or dead cells. For a cell viability stain, one can use Calcein Blue AM. The difference between Calcein Blue and Calcein Blue AM is the methyl-iminodiaceitc acid group that chelates the divalent metal has acetoxymethyl esters on it in Calcein Blue AM. The esters are cleaved inside of viable cells, but remain on the molecule in the absence of active esterases. So, if you use M1255 it will stain all the cell whether alive or dead.
M1255
Calcein Blue
Powder
Sinônimo(s):
4-Methylumbelliferone-8-methyliminodiacetic acid
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R$ 648,00
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R$ 3.413,00
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1 G
R$ 648,00
10 G
R$ 3.413,00
About This Item
Fórmula empírica (Notação de Hill):
C15H15NO7
Número CAS:
Peso molecular:
321.28
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47
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Nome do produto
Calcein Blue, fluorescent dye
Nível de qualidade
Formulário
powder
técnica(s)
titration: suitable
cor
white to light yellow
solubilidade
1 M NaOH: 50 mg/mL
fluorescência
λex 322 nm; λem 445 nm in methanol
adequação
suitable for indicator for EDTA titration
aplicação(ões)
diagnostic assay manufacturing
hematology
histology
temperatura de armazenamento
room temp
cadeia de caracteres SMILES
CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
InChI
1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
chave InChI
RJPSHDMGSVVHFA-UHFFFAOYSA-N
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Descrição geral
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
Aplicação
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Gloves
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Is Calcein Blue identical to Calcein Blue AM, and if not, what is the difference? Would it be compatible in a flourescent microscopy assay looking at %viability of THP-1 cells?
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