C1359

Calcein AM solution

≥90% purity (HPLC), solution

• Fórmula empírica (Notação de Hill): C₄₆H₄₆N₂O₂₃
• Número CAS: 148504-34-1
• Peso molecular: 994.86
• Número CE: 200-664-3
• Número MDL: MFCD05861516
• Código UNSPSC: 12171500
• ID de substância PubChem: 24892405
• NACRES: NA.47

Propriedades

• Nome do produto: Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
• Nível de qualidade: 100
• Ensaio: ≥90% (HPLC)
• Formulário: solution
• concentração: 4 mM in DMSO
• técnica(s): titration: suitable
• cor: colorless; white to off-white
• λmax: 491-497 nm
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• chave InChI: XKFSBWQWNMZWFA-UHFFFAOYSA-N

Descrição

Aplicação

In cells, calcein AM solution has been used in calcein-AM dye transfer assay.[1] It has been used for the staining of cells to study quantification of cell-migration.[2] It has been used to study cytotoxic effects of drugs in cells.[3]

Ações bioquímicas/fisiológicas

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.[4]

Fluorescent cell permeable derivative of calcein.

forma física

Solution in 0.4% Calcein AM and 99.6% DMSO.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): 185.0 °F - closed cup
• Ponto de fulgor (°C): 85 °C - closed cup
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)