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L7018

Sigma-Aldrich

Lipopolysaccharides from Pseudomonas aeruginosa 10

purified by trichloroacetic acid extraction

Sinônimo(s):

LPS

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About This Item

Número MDL:
MFCD00131520
Código UNSPSC:
12352201
NACRES:
NA.25
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

Pseudomonas aeruginosa (10)

Nível de qualidade

200

Formulário

lyophilized powder

purificado por

trichloroacetic acid extraction

Impurezas

1-10% Protein (Lowry)

cor

white to yellow

solubilidade

water: 4.90-5.10 mg/mL, hazy to turbid, colorless to light yellow

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

This product is TCA extracted from Pseudomonas aeruginosa serotype 10. The source strain is ATCC 27316.

Aplicação

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Ações bioquímicas/fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparo

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Outras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000198952
0000198953
0000193239
0000193239
0000198952

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