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L2880

Sigma-Aldrich

Lipopolissacarídeos

Lipopolysaccharides from Escherichia coli O55:B5

purified by phenol extraction

Sinônimo(s):

LPS

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10 MG
R$ 929,00
25 MG
R$ 1.665,00
100 MG
R$ 3.604,00
500 MG
R$ 13.829,00

R$ 929,00

Disponível para enviar em10 de abril de 2025Detalhes

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10 MG
R$ 929,00
25 MG
R$ 1.665,00
100 MG
R$ 3.604,00
500 MG
R$ 13.829,00

About This Item

Número CAS:
93572-42-0
Número CE:
297-473-0
Número MDL:
MFCD00164401
Código UNSPSC:
12352201
NACRES:
NA.25

R$ 929,00

Disponível para enviar em10 de abril de 2025Detalhes

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fonte biológica

Escherichia coli (O55:B5)

Nível de qualidade

300

Formulário

lyophilized powder

purificado por

phenol extraction

técnica(s)

cell based assay: suitable

Impurezas

<3% Protein (Lowry)

cor

off-white to tan

solubilidade

water: soluble

aplicação(ões)

cell analysis
clinical research
life science and biopharma

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Procurando produtos similares? Visita Guia de comparação de produtos

Categorias relacionadas

Descrição geral

Os lipopolissacarídeos (LPS) são glicolipídeos,[1] uma combinação de unidades de carboidratos ligadas a ácidos graxos simples ou múltiplos, encontrados na parede celular de bactérias Gram-negativas.[2] Os LPS são um componente essencial da membrana externa e sua estrutura é composta pelo lipídio A, um fosfolipídio à base de glucosamina, um oligossacarídeo de núcleo curto e um antígeno O, um polissacarídeo distal.[3]

Aplicação

Foi usado o lipopolissacarídeo de Escherichia coli O55:B5:
  • para estimular hepatócitos e células não parenquimatosas[4]
  • para estimular o baço e estudar a expressão do gene Malitaf no peixe Megalobrama amblycephala[5]
  • como parte do modelo de hepatite fulminante de concanavalina A (ConA) e D-galactosamina/lipopolissacarídeo (D-GalN/LPS) para estudar o efeito do composto natural hesperetina[6]
  • para verificar a viabilidade celular de células-tronco mesenquimais derivadas de vilosidades coriônicas humanas (hCMSCs) por meio do ensaio de metil-tiazolil-tetrazólio (MTT)[7]
  • para induzir a toxicidade dos oligodendrócitos avaliar os efeitos neuroprotetores de alguns compostos[8]
  • para induzir a sarcopenia em ratos avaliar os efeitos antioxidantes da hidroxiapatita modificada por superfície hidrofóbica carregada com curcumina[9]
Os lipopolissacarídeos (LPSs) são componentes característicos da parede celular de bactérias gram negativas. Os LPS e sua porção lipídica A estimulam as células do sistema imunológico inato através do receptor do tipo Toll 4 (TLR4), um membro da família de proteínas do receptor do tipo Toll 4 que reconhece patógenos comuns associados a padrões moleculares (PAMPs).

Ações bioquímicas/fisiológicas

Os lipopolissacarídeos (LPS) criam uma barreira protetora de permeabilidade que bloqueia a entrada de antibióticos e sais biliares, o que torna as bactérias Gram-negativas inatamente resistentes. Os LPS estão envolvidos na patogenicidade bacteriana, pois o sistema imunológico do hospedeiro encontra principalmente o componente LPS da bactéria. Os LPS são uma endotoxina e um antígeno altamente imunogênico que pode melhorar o sistema imunológico. O lipídio A dos LPS estimula as células hospedeiras por meio dos receptores toll-like 4 (TLR4).[1]

Outras notas

Vencedor do Prêmio CiteAb 2024 para Fornecedor de Sucesso em Pesquisa sobre Parkinson′
Para a reconstituição ideal dos produtos de LPS, um vórtex intensivo é necessário antes do uso, independentemente do tipo de recipiente. Certifique-se de que a concentração da solução de armazenamento seja de pelo menos 1 mg/ml. Embora os recipientes de borossilicato (silanizados) sejam recomendados, também podem ser usados recipientes plásticos de polipropileno (PP).
Para mais informações sobre nossa linha de Bioquímicos, preencha este formulário.
Para obter uma compreensão abrangente de nossa ampla gama de lipopolissacarídeos para sua pesquisa, recomendamos que você visite nossa página de categoria de carboidratos.

Pictogramas

Skull and crossbones
GHS06

Palavra indicadora

Danger

Frases de perigo

H300

Declarações de precaução

P264 - P270 - P301 + P310 - P405 - P501

Classificações de perigo

Acute Tox. 2 Oral

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000428249
0000428249
0000428255
0000428255
0000428250

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Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Yun Feng et al.
Stem cell research & therapy, 11(1), 2-2 (2020-01-05)
ALI/ARDS is the major cause of acute respiratory failure in critically ill patients. As human chorionic villi-derived MSCs (hCMSCs) could attenuate ALI in the airway injury model, and liraglutide, glucagon-like peptide 1 (GLP-1) agonist, possesses anti-inflammatory and proliferation promotion functions
Ya-Jyun Liang et al.
Antioxidants (Basel, Switzerland), 10(4) (2021-05-01)
Oxidative stress and later-induced chronic inflammation have been reported to play an important role on the progression of sarcopenia. Current treatments for sarcopenia are mainly administered to patients whom sarcopenia already developed. However, there has been no promising results shown
A liver microphysiological system of tumor cell dormancy and inflammatory
responsiveness is affected by scaffold properties
Clark AM
Cancer Discovery null
Outer membrane permeability and antibiotic resistance
Delcour A H, et al.
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1794(5) , 808-816 (2009)
Promising neuroprotective effects of ?-caryophyllene against LPS-induced oligodendrocyte toxicity: A mechanistic study
Askari V R and Shafiee-Nick R
Biochemical Pharmacology, 159, 154-171 (2019)
Exibir todos os documentos relacionados

Artigos

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

1 Review This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. I recently purchased this item and I would like to understand how lethal/toxic/dangerous is for humans once diluted to 0.5mg/ml. I would need to inject ip fish with a certain volume from that stock solution.

    1 answer

    1. Toxicological information, when available, is provided in section 11 of the safety data sheet. It is unclear which fish model is being used to study toxicity. Consulting scientific literature is recommended for these details. Below is some information that may be useful to address the question.

      https://www.sigmaaldrich.com/sds/sigma/l2880?userType=anonymous

      https://www.tandfonline.com/doi/abs/10.1080/10934529.2012.695254

      https://www.mdpi.com/1420-3049/19/2/2390

      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides?srsltid=AfmBOoo1e2mKmwy-vXjZvAmV-Epz80sS4TfN11C2ShOHlc0FPWkb9_2n

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Hi, Can you provide the information regarding molecular weight of this LPS?

    1 answer

    1. The molecular weight of this product has not been determined. In general, the molecular weight of native LPS may range from 1 – 4 million Daltons or higher. When treated with SDS and heat, the molecular weight of LPS may range from 50 to 100 kDa.

      Helpful?

  5. What is the number of acetyl groups attached to the lipid A moiety?

    1 answer

    1. The acyl chain length and number of acyl groups may vary between bacterial species. Certain researchers claim there are 6 acyl groups for LPS from this strain of bacteria. Please refer to those peer reviewed papers below for more information.

      https://www.sciencedirect.com/science/article/pii/S0939641122002338#:~:text=%5B14%5D%2C%20%5B42%5D,in%20a%20single%20LPS%20structure.

      https://pubmed.ncbi.nlm.nih.gov/16934960/

      Helpful?

  6. Can you provide information on the solubility and solution stability of this product?

    1 answer

    1. LPS products in solution are expected to be stable in phosphate buffered saline at a concentration of 1 mg/mL when stored at 4 °C for at least one month. For long-term storage, it is recommended to use frozen aliquots. In order to prevent LPS binding to glass, it is advisable to store the solution in silanized containers. If glass containers are used, it is recommended to vortex the solutions for at least 30 minutes to dissolve any bound LPS.

      Helpful?

  7. Does the bacterial strain have specific information available, such as whether it originates from a private collection or if there is an associated ATCC number?

    1 answer

    1. Unfortunately, the specific strains used to make L2880 are considered proprietary.

      Helpful?

  8. hello, I was wondering what the best way to reconstitute this LPS is

    1 answer

    1. LPS products can be reconstituted in phosphate buffered saline at 1 mg/mL.

      Helpful?

  9. Which LPS grade should be used to induce neurotoxicity in rats?

    1 answer

    1. There is no Lipopolysaccharide product offered specifically for the induction of neurotoxicity in rats. Escherichia coli strain O55:B5 has been used to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of certain compounds. In addition, Escherichia coli strain O111:B4 has been cited in the literature as suitable for LPS-induced neurotoxicity. For use with animal models, LPS purified by gel filtration is most commonly used to eliminate the risk of any residual solvents.

      Please see the links below to review the E. coli strains O111:B4 and O55:B5 that have been purified by gel filtration:
      Lipopolysaccharides – Glycobiology
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides
      Lipopolysaccharides – selection table
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Helpful?

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