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L4895

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

Sinônimo(s):

WGA, Wheat germ agglutinin

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

conjugado

FITC conjugate

Nível de qualidade

200

forma

lyophilized powder

potência

<50 μg/mL agglutination activity

composição

Protein, ~50% Lowry

Extensão da rotulagem

~2 mol FITC per mol protein

temperatura de armazenamento

2-8°C

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Descrição geral

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Aplicação

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence
  • to stereotaxically inject into mice for neuronal tracing
  • in immunohistochemistry for the analysis of myocytes

Ações bioquímicas/fisiológicas

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Embalagem

Package size based on protein content.

forma física

Contains phosphate buffer salts

Nota de preparo

Conjugates are prepared from affinity purified lectin.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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