L0382

Lipase from porcine pancreas

Type VI-S, ≥20,000 units/mg protein, lyophilized powder

• Sinônimo(s): PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Número CAS: 9001-62-1
• Número da licença da enzima: 3.1.1.3 (BRENDA, IUBMB)
• Número CE: 232-619-9
• Número MDL: MFCD00131509
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• tipo: Type VI-S
• forma: lyophilized powder
• atividade específica: ≥20,000 units/mg protein
• composição: Protein, 40-70%
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• chave InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Descrição

Descrição geral

Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwich domain. PPL belongs to a subclass of the carboxylesterases family.

Aplicação

Lipase from porcine pancreas has been used:

• in lipase inhibition assay with plant extracts
• in in vitro digestion gelatinized starch/fiber mixtures
• with simulated intestinal fluid to mimic the digestive tract condition of by L. fermentum PC1 in fermented oats

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Ações bioquímicas/fisiológicas

Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms. PPL is cost-effective and is useful in biotransformation reactions. It displays high selectivity, and catalytic activity. PPL is also used in the detergent formulation for removing oil stains from clothes.

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Definição da unidade

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Nota de análise

Protein determined by biuret.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)