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I1408

Sigma-Aldrich

IMAC-Select Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Sinônimo(s):

Affinity Gel

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

forma

(1:1 suspension in a 30% ethanol solution)

prazo de validade

1 yr

técnica(s)

affinity chromatography: suitable

Impurezas

≤0.08 Metal Contamination (μmol/ml packed gel)

matriz

6% beaded agarose

capacidade

10-30 μmol/mL binding capacity (Cu+2)

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Categorias relacionadas

Aplicação

An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.

forma física

1:1 suspension in a 30% ethanol solution

Nota de preparo

The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Outras notas

Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Declarações de precaução

P210 - P233 - P240 - P241 - P242 - P243

Classificações de perigo

Flam. Liq. 3

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

120.2 °F - closed cup

Ponto de fulgor (°C)

49 °C - closed cup

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Kris Gevaert et al.
Methods in molecular biology (Clifton, N.J.), 527, 219-227 (2009-02-26)
We present a gel-free proteomics procedure for the specific isolation of phosphorylated peptides from whole proteome digests. Central is the use of diagonal, reverse-phase chromatography which consists of two consecutive reverse-phase peptide separations with a modification step in between. The
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
The Journal of biological chemistry, jbc-M111 (2012)
Yishai Levin et al.
Journal of proteomics, 73(3), 689-695 (2009-11-10)
In order to exploit human blood as a source of protein disease biomarkers, robust analytical methods are needed to overcome the inherent molecular complexity of this bio-fluid. We present the coupling of label-free SAX chromatography and IMAC to a data-independent
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
Test, jbc-M111 (2012)
The hantavirus glycoprotein G1 tail contains dual CCHC-type classical zinc fingers
Estrada DF, et al.
The Journal of Biological Chemistry, 284(13), 8654-8660 (2009)
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