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H0537

Millipore

HIS-Select® HF Nickel Affinity Gel

Sinônimo(s):

Ni-NTA HF resin

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56
Preço e disponibilidade não estão disponíveis no momento.

Formulário

suspension

Nível de qualidade

200

técnica(s)

affinity chromatography: suitable

Matriz

Highly cross-linked 6% Beaded Agarose

capacidade

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Aplicação

Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of HIS TAG® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Características e benefícios

  • High selectivity for higher purity
  • Unique non-charged hydrophilic linkage reduced non-specific binding
  • Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
  • Highly cross-linked agarose matrix allows for purification under higher pressures
  • Binding under denaturing or non-denaturing conditions
  • One-step purification

forma física

1:1 suspension in a 30% ethanol solution

Nota de preparo

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Informações legais

FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2

Órgãos-alvo

Eyes,Central nervous system, Respiratory Tract

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

88.0 °F

Ponto de fulgor (°C)

31.1 °C

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análise (COA)

Lot/Batch Number
SLCQ5051
SLCL9242
SLCG1131
SLCB1729
SLBR9492V

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