H9789

Anti-HMGB2 (HMG2) antibody produced in rabbit

affinity isolated antibody

• Sinônimo(s): Anti-High mobility group box 2, Anti-High mobility group protein 2
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: rat, human, mouse
• embalagem: antibody small pack of 25 μL
• técnica(s): immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.; indirect immunofluorescence: 1-2 μg/mL using fixed PC12 cultured cells.; western blot: 2-4 μg/mL using PC12 whole cell lysates.
• nº de adesão UniProt: P26583
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HMGB2(3148) ; mouse ... Hmgb2(97165) ; rat ... Hmgb2(29395)

Descrição

Descrição geral

The high mobility group (HMG) proteins are subdivided into 3 families based on their functional sequence motif characteristic: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) and the HMGA (formerly HMG-I/Y/C) family. The HMGB protein family, including HMGB1, HMGB2, and HMGB3, are highly conserved (more than 80% amino acid identity), and indistinguishable in their biochemical properties. The high-mobility group box 2 (HMGB2) is a ~25 kDa protein of 209 amino acids that consists of two homologous HMG-boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMGB2 is restricted mainly to lymphoid organs and testes, although it is widely expressed in embryogenesis.

Imunogênio

synthetic peptide corresponding to amino acid 165-181 of human HMGB2, conjugated to KLH. The corresponding sequence is conserved in mouse and differs by one amino acid in rat.

Aplicação

Anti-HMGB2 (HMG2) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol.

Ações bioquímicas/fisiológicas

The high-mobility group box 1 and 2 (HMGB 1 & 2) proteins participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signalling.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)