This product should be stored in room temperature in powdered format.
H3149
Heparin sodium salt from porcine intestinal mucosa
Heparin sodium salt from porcine intestinal mucosa
Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
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About This Item
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
Porcine intestinal mucosa
Nível de qualidade
tipo
Grade I-A
linha de produto
BioReagent
Formulário
powder
atividade específica
≥180 USP units/mg
técnica(s)
analytical sample preparation: suitable
cell culture | mammalian: suitable
cor
beige
solubilidade
H2O: 50 mg/mL
aplicação(ões)
cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation
cadeia de caracteres SMILES
[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O
InChI
1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-
chave InChI
JRTRSJGZMRQDHI-UHFFFAOYSA-N
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Descrição geral
Heparin is mainly responsible for the delay in the coagulation of blood. It enhances the antithrombin-mediated inactivation of proteases in the coagulation pathway.[1]
Heparin sodium, a sulfated polysaccharide from the glycosaminoglycan family, plays a vital role in cell culture, cancer biology, and drug discovery research. Its impact on protein and cell interactions makes it valuable for studying molecular and cellular processes, tumor modeling, exploring binding interactions, and as a cell culture supplement to support cell viability.
Aplicação
- Heparin sodium salt from porcine intestinal mucosa has been used in sLRP (soluble circulating low density lipoprotein receptor-related protein-1) purification from human plasma.[2]
- It has been used as a supplement in M199 medium for culturing of human umbilical vein endothelial cells.[3]
- It has been used as an anticoagulant agent during isolation of blood.[4]
Características e benefícios
- High-purity compound suitable for a wide variety of research applications
Outras notas
For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
produto comparável
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Certificados de análise (COA)
Lot/Batch Number
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Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Factor Xa and thrombin, but not factor VIIa, elicit specific cellular responses in dermal fibroblasts.
Bachli EB et al.
Journal of Thrombosis and Haemostasis, 1, 1935-1935 (2003)
Effects of T-lymphocyte depletion on muscle fibrosis in the mdx mouse.
Morrison J, et al.
The American Journal of Pathology, 166, 1701-1701 (2005)
Structure of the antithrombin-binding site in heparin.
Lindahl U et al.
Proceedings of the National Academy of Sciences of the USA, 76, 3198-3202 (1979)
Mohamed Abdouh et al.
Journal of experimental & clinical cancer research : CR, 36(1), 113-113 (2017-09-01)
Horizontal transfer of malignant traits from the primary tumor to distant organs, through blood circulating factors, has recently become a thoroughly studied metastatic pathway to explain cancer dissemination. Recently, we reported that oncosuppressor gene-mutated human cells undergo malignant transformation when
Keisuke Yoshida et al.
Nature communications, 9(1), 3885-3885 (2018-09-27)
The majority of histones are replaced by protamines during spermatogenesis, but small amounts are retained in mammalian spermatozoa. Since nucleosomes in spermatozoa influence epigenetic inheritance, it is important to know how histones are distributed in the sperm genome. Conflicting data
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What is the storage condition of H3149 powder?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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H3149-10KU heparin sodium salt is steril??
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The product is not sterile. Prepared solutions may be filter sterilized.
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please, could you explain me if this H3149 is one of 'high molecolar weight heparin'? thanks
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This product is a cell culture tested porcine heparin with a molecular weight range of 15-19 kDa. This material is considered a "high molecular weight heparin." According to the literaure, low molecular weight heparins are those with a molecular wieght of less that 8000 Da. See the link below to review a publication that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141002/Helpful?
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Can this material be diluted to 10,000 U/mL and stored at 2C to 8C? Do you have stability data at this temperature for lower concentrations?
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Yes, this product remains biologically active in dilute solutions. Stock solutions prepared at 50 mg/mL in water are stable for up to 2 years at 2 - 8°C, when filter sterilized. According to literature, heparin diluted in physiological saline at a concentration of 10 to 100 units/mL is stable for at least 35 days when stored at 2 - 8°C. Please see the link below to review the datasheet for an identical product, H3393, which may be applied to this item
H3393 Datasheet
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/608/h3393pis.pdfHelpful?
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Can you recommend a suitable heparin product for mouse heart perfusion?
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Our available heparin products have not undergone specific testing for mouse heart perfusion. However, you may find H3149 of interest as it has been cited in the following publication for this intended application: "Isolation and Primary Culture of Mouse Aortic Endothelial Cells" published in J Vis Exp. 2016; (118): 52965 (PMID: 28060318).
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Is the heparin sodium salt (H3149, CAS 9041-08-1) unfractionated or fractionated? How does this classification relate to its molecular weight?"
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The heparin sodium salt (H3149, CAS 9041-08-1) is unfractionated. This classification is reflected in its molecular weight, which ranges approximately from 9,000 to 21,000, with the majority falling between 17,000-19,000. As confirmed by our product and as indicated in the USP monograph, our heparin is unfractionated. This differs from fractionated Heparin, also known as Low Molecular Weight Heparin, which has a much lower molecular weight. According to the DBR, 3rd ed., p. 342, the molecular weight of our heparin is approximately 16,000-17,000.
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Is it a different substance from heparan sulfate?
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Heparin is not equivalent to heparin sulfate. Please see the link below to describe the differences:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/what-is-heparinHelpful?
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Can I dissolve the heparin in filtered (0.22 um) nuclease free water at a concentration of 50mg/ml? Does it correspond to 5000 IU/ml? Then, I can use 15-30 IU/ ml of blood to avoid its coagulation, is it correct?
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As mentioned on the Product Detail Page, this product is soluble at 50 mg/mL in water. The actual activity is reported on the lot-specific Certificate of Analysis. This value should be used in determining the activity of the prepared solution. The activity specification for this product is >/=180 USP units/mg. For example, assuming an activity value of 200 units/mg, a prepared solution of 50 mg/ml will have an activity of 5000 units/ml. Yes, typically, 15 - 30 units/ml is suitable for use as an anticoagulant.
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