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Documentos Principais

GE28-9574-99

TALON® Superflow™

Cytiva 28-9574-99, pack of 10 mL

Sinônimo(s):

TALON Affinity Resin

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About This Item

Código UNSPSC:
23151817
NACRES:
NA.56
Preço e disponibilidade não estão disponíveis no momento.

ligand

TALON® Superflow Cobalt-IMAC

embalagem

pack of 10 mL

fabricante/nome comercial

Cytiva 28-9574-99

Matriz

6% cross-linked agarose

tamanho de partícula

60-160 μm

cleaning in place

2-14(Co2+-stripped medium)

Faixa de trabalho

3-12(Co2+-stripped medium)

capacidade

≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media.

Características e benefícios

  • Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

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Protocolos

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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