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GE17-5442-02

Ficoll®-Paque Premium

Cytiva 17-5442-02, pack of 6 × 100 mL

Sinônimo(s):

Ficoll density gradient medium, cell culture media

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About This Item

Código UNSPSC:
12352201
NACRES:
NA.25
Preço e disponibilidade não estão disponíveis no momento.

Formulário

liquid

embalagem

pack of 6 × 100 mL

fabricante/nome comercial

Cytiva 17-5442-02

cor

Colorless to slightly yellow

faixa de pH

5.5-7.5

Categorias relacionadas

Descrição geral

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

Aplicação

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

Características e benefícios

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Ficoll is a registered trademark of Cytiva

Código de classe de armazenamento

12 - Non Combustible Liquids

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Certificados de análise (COA)

Lot/Batch Number

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Xin Ni et al.
PloS one, 12(9), e0184773-e0184773 (2017-09-15)
Inflammation has been shown to play an important role in the mechanisms involved in the pathogenesis of hypertension. Connexins (Cxs)-based gap junction channels (GJCs) or hemichannels (HCs) are involved in the maintenance of homeostasis in the immune system. However, the
Avanish K Varshney et al.
PloS one, 13(1), e0190537-e0190537 (2018-01-25)
Staphylococcus aureus can cause devastating and life-threatening infections. With the increase in multidrug resistant strains, novel therapies are needed. Limited success with active and passive immunization strategies have been attributed to S. aureus immune evasion. Here, we report on a

Artigos

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads facilitate Percoll gradient monitoring in biological separations.

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Protocolos

Recommended Standard Method for Isolating Mononuclear Cells

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Conteúdo relacionado

Applications of Percoll In Blood Cells

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

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