GE17-5130-02

GSTrap^™ Fast Flow

Cytiva 17-5130-02, pack of 2 × 1 mL

• Código UNSPSC: 23151817
• NACRES: NA.25

Propriedades

• ligand: glutathione and 10-carbon linker arm
• embalagem: pack of 2 × 1 mL
• fabricante/nome comercial: Cytiva 17-5130-02
• Parâmetros: <4 mL/min flow rate; 42 psi
• tamanho do leito: 7 mm × 25 mm
• volume do leito: 1 mL
• D.I. da coluna: 7 mm
• matriz: highly cross-linked 4% agarose
• diâmetro médio: 90 μm
• cleaning: 3-12
• working range: 3-12
• capacidade: >10 mg/mL binding capacity

Descrição

Descrição geral

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Aplicação

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrapTM FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione SepharoseTM 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTATM design or FPLCTM System.

Características e benefícios

• Fast and simple one-step purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast FLow provides good fLow properties.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids