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GE17-0709-10

DEAE Sepharose Fast Flow

Cytiva 17-0709-10, pack of 25 mL

Sinônimo(s):

Anion exchange purification

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

diethylaminoethyl

descrição

Ion Exchanger Type (value)

embalagem

pack of 25 mL

fabricante/nome comercial

Cytiva 17-0709-10

matriz

6% cross-linked agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning in place

2-14

working range

2-12

adequação

suitable for bioprocess medium

Descrição geral

DEAE Sepharose Fast FLow is a weak anion exchanger based on the well established Sepharose Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Aplicação

DEAE Sepharose Fast FLow is part of the Sepharose Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. DEAE Sepharose Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with DEAE Sepharose Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, DEAE Sepharose Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características e benefícios

  • Well-proven weak anion exchanger developed for industrial downstream processes
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

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Artigos

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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