GE17-0729-01
SP Sepharose™ Fast Flow
Cytiva 17-0729-01, pack of 300 mL
Sinônimo(s):
SP Sepharose, Sepharose Fast Flow Column
About This Item
Produtos recomendados
ligand
sulphopropyl
descrição
Ion Exchanger Type (value)
embalagem
pack of 300 mL
fabricante/nome comercial
Cytiva 17-0729-01
matriz
6% cross-linked agarose
tamanho de partícula
45-165 μm
diâmetro médio
90 μm
cleaning in place
3-14
working range
4-13
capacidade
70 mg binding capacity(ribonuclease A/ml medium)
adequação
suitable for bioprocess medium
Categorias relacionadas
Descrição geral
SP Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with sulphopropyl (Q) strong cation exchange groups. SP Sepharose™ Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP Sepharose™ Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.
As member of the BioProcess media range, SP Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Características e benefícios
- Well-proven strong cation exchanger developed for industrial downstream processes.
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Armazenamento e estabilidade
Nota de análise
Informações legais
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
Certificados de análise (COA)
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Protocolos
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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