G6532

Anti-GroEL antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinônimo(s): Anti-GroEL, GroEL Antibody - Anti-GroEL antibody produced in rabbit - Tested Applications: immunoblotting 1:80,000,indirect ELISA 1:40,000-1:80,000 - Sigma Aldrich G6532, Groel Antibody
• Código UNSPSC: 12352203
• NACRES: NA.46

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: IgG fraction of antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• reatividade de espécies: E. coli
• técnica(s): indirect ELISA: 1:40,000-1:80,000; western blot: 1:80,000
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: Escherichia coli ... groEL(913705) , groEL(913705) ; Escherichia coli K12 ... Grol(948665)

Descrição

Descrição geral

GroEL protein belongs to the folding chaperonin family. It is a 58.3 kDa protein with apical, intermediate and equatorial functional domains.

Especificidade

The antibody does not cross-react with GroES and bovine HSP 70.

Imunogênio

purified recombinant GroEL produced in E. coli.

Aplicação

Anti-GroEL antibody produced in rabbit has been used as positive control in histology and immunohistochemistry of Dirofilaria immitis, nematode worms and in the western blot analysis of the HeLa cells.

Ações bioquímicas/fisiológicas

GroEL, together with GroES, in the presence of ATP, assists in protein folding. Co-expression of aldehyde dehydrogenase with GroEL/GroES promotes folding and higher production of protein in soluble form. GroES and GroEL bind to the protein substrate, enclosing it in a cage like fold. The target protein after folding gets dissociated from the cage and is accompanied with hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)