We were trying to use this as a protein standard and found it to be a heterogeneous mess. Not sure what all is in here, but its a lot more than betagalactosidase
G6008
β-Galactosidase from Escherichia coli
β-Galactosidase from Escherichia coli
1 of 1 reviewers received a sample product or took part in a promotion
Grade VI, lyophilized powder, ≥250 units/mg protein
Sinônimo(s):
β-D-Galactoside galactohydrolase, Lactase
Faça loginpara ver os preços organizacionais e de contrato
Selecione um tamanho
1000 UNITS
R$ 824,00
5000 UNITS
R$ 2.354,00
10000 UNITS
R$ 4.057,00
R$ 824,00
Check Cart for Availability
Selecione um tamanho
Alterar visualização
1000 UNITS
R$ 824,00
5000 UNITS
R$ 2.354,00
10000 UNITS
R$ 4.057,00
About This Item
R$ 824,00
Check Cart for Availability
Produtos recomendados
fonte biológica
Escherichia coli
Nível de qualidade
tipo
Grade VI
Formulário
lyophilized powder
atividade específica
≥250 units/mg protein
peso molecular
465 kDa
não contém
BSA as extender
composição
Protein, ≥50% biuret
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Procurando produtos similares? Visita Guia de comparação de produtos
Descrição geral
β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.[1]
Aplicação
Ações bioquímicas/fisiológicas
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop[4].
propriedades físicas
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Definição da unidade
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
forma física
Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol
Inibidor
substrato
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
Não está vendo a versão correta?
Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
David P Klebl et al.
Frontiers in molecular biosciences, 9, 945772-945772 (2022-08-23)
Advances in single particle cryo-EM data collection and processing have seen a significant rise in its use. However, the influences of the environment generated through grid preparation, by for example interactions of proteins with the air-water interface are poorly understood
K Kato et al.
Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
G R CRAVEN et al.
The Journal of biological chemistry, 240, 2468-2477 (1965-06-01)
D C Young et al.
Analytical biochemistry, 215(1), 24-30 (1993-11-15)
Bacterial beta-galactosidase is one of several reporter enzymes used in studying the transcriptional activity of eukaryotic promoters. Although it is one of the easiest and least expensive enzymes to assay, its use has been limited because of its low sensitivity
Orit Redy-Keisar et al.
Nature protocols, 9(1), 27-36 (2013-12-07)
This protocol describes the synthesis of modular turn-ON QCy7-based probes for the detection of biologically relevant analytes, such as hydrogen peroxide, ubiquitous sulfhydryl and β-galactosidase. The probes presented herein are prepared through a simple procedure that involves the preliminary alkylation
Active Filters
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
